rabbit anti oprl1 Search Results


rabbit anti oprl1  (Bioss)
94
Bioss rabbit anti oprl1
Rabbit Anti Oprl1, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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732 anti lox1 orl1 golocdacimab  (MedChemExpress)
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∘ c  (Proteintech)
93
Proteintech ∘ c
∘ C, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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8-ohdg polyclonal antibody  (Bioss)
96
Bioss 8-ohdg polyclonal antibody
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cdc2/cdk1 polyclonal antibody  (Bioss)
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Bioss cdc2/cdk1 polyclonal antibody
Cdc2/Cdk1 Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd16 polyclonal antibody  (Bioss)
94
Bioss cd16 polyclonal antibody
Cd16 Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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prrsv m protein polyclonal antibody  (Bioss)
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Bioss prrsv m protein polyclonal antibody
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collagen 7 polyclonal antibody  (Bioss)
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Bioss collagen 7 polyclonal antibody
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goat-anti-rabbit exoab antibody kit exoab-kit-1  (System Biosciences Inc)
90
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Goat Anti Rabbit Exoab Antibody Kit Exoab Kit 1, supplied by System Biosciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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polyclonal anti orl1  (ProSci Incorporated)
86
ProSci Incorporated polyclonal anti orl1
Polyclonal Anti Orl1, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tris buffered saline  (Proteintech)
96
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rabbit anti-gpr20 polyclonal  (Thermo Fisher)
90
Thermo Fisher rabbit anti-gpr20 polyclonal
Validated hits from secondary and tertiary screens of ciliogenesis Secondary screen hits are listed (n=68, validated from a total of 194 functional candidates) that had a significant effect on ciliogenesis in mouse mIMCD3 cells (given by robust z scores if z cilia < z cilia cutoff = −1.34, calculated as the median z of positive controls) for n> =1/4 individual siRNAs duplexes in duplicate. Tertiary screen hits in human hTERT-RPE1 cells for defects in cilia number and/or length (n=37/68, using cut-offs of z <−2 or z >+2) for siRNA pools in duplicate. Human and mouse gene symbols, mouse gene IDs, known or inferred protein function and selected OMIM annotations are also listed.
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Image Search Results


Validated hits from secondary and tertiary screens of ciliogenesis Secondary screen hits are listed (n=68, validated from a total of 194 functional candidates) that had a significant effect on ciliogenesis in mouse mIMCD3 cells (given by robust z scores if z cilia < z cilia cutoff = −1.34, calculated as the median z of positive controls) for n> =1/4 individual siRNAs duplexes in duplicate. Tertiary screen hits in human hTERT-RPE1 cells for defects in cilia number and/or length (n=37/68, using cut-offs of z <−2 or z >+2) for siRNA pools in duplicate. Human and mouse gene symbols, mouse gene IDs, known or inferred protein function and selected OMIM annotations are also listed.

Journal: Nature cell biology

Article Title: An siRNA-based functional genomics screen for the identification of regulators of ciliogenesis and ciliopathy genes

doi: 10.1038/ncb3201

Figure Lengend Snippet: Validated hits from secondary and tertiary screens of ciliogenesis Secondary screen hits are listed (n=68, validated from a total of 194 functional candidates) that had a significant effect on ciliogenesis in mouse mIMCD3 cells (given by robust z scores if z cilia < z cilia cutoff = −1.34, calculated as the median z of positive controls) for n> =1/4 individual siRNAs duplexes in duplicate. Tertiary screen hits in human hTERT-RPE1 cells for defects in cilia number and/or length (n=37/68, using cut-offs of z <−2 or z >+2) for siRNA pools in duplicate. Human and mouse gene symbols, mouse gene IDs, known or inferred protein function and selected OMIM annotations are also listed.

Article Snippet: The following antibodies were used with the indicated titres for immunofluorescent staining: rabbit anti-PRP6 (Santa Cruz sc-48786) 1:100, mouse anti-PRPF8 (Santa Cruz sc-55534) 1:100, rabbit anti-PRPF8 (Santa Cruz sc-030207) 1:100, goat anti-PRPF31 (Abnova PAB7154) 1:100, PIBF1/CEP90 (Novus NBP2-19823) 1:100, rabbit anti-PLK4 (Cell Signalling Technologies 3258) 1:100, rabbit anti-GPR20 polyclonal (Thermo Scientific, PA3-068) 1:100, rabbit anti-SREB3 (Novus Biologicals NBP1-71064) 1:100, rabbit anti-MAS1 (Novus Biologicals NBP1-78444) 1:100, rabbit anti-HTR1B (Novus Biologicals NBP1-01013) 1:100, rabbit anti-P2RY14 (Novus NBP1-70970) 1:100, rabbit anti-CRHR2 (Novus NBP1-00767) 1:100, rabbit anti-OPRL1 (Abcam ab66219) 1:50, rabbit anti-C21orf2 (GeneTex [N1C2], GTX119046) 1:100, mouse anti-acetylated alpha tubulin (Sigma Aldrich clone 6-11 B1 T6793) 1:1000, rat anti-ZO1 (1:400, Santa Cruz), rabbit anti-β-catenin (1:400, BD Bioscience), rabbit anti-gamma tubulin (AbCam ab11317) 1:1000, rabbit anti-ARL13B (Proteintech 17711-1-AP) 1:1000, mouse anti-GT335 monoclonal (Enzo Life Sciences ALX-804-885-C100) 1:1000 and mouse anti-FLAG (Sigma F3165) 1:1000.

Techniques: Functional Assay, Activity Assay, Modification, Binding Assay

(a) Localisation of selected indicated GPCRs (green) to proximal or basal regions of primary cilia (polyglutamylated α-tubulin; red) in differentiated SH-SY5Y neuronal cells. Magnified insets for selected cells (white arrowheads) are shown in white frames. Scale bar = 10μm. ( b ) Immunofluorescence (IF) labelling of MAS1 and OPRL1 (green) in the ciliary regions of photoreceptor cells (CR) (red; yellow arrowheads in magnified insets) in adult mouse retina. Abbreviations: GCL, ganglion cell layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer. Scale bar = 50μm ( c ) High magnification immunofluorescence for GPCRs (green) and centrin-3 (red) reveals localisation of GPR20, SREB3, and MAS1 at the adjacent centriole and the basal body (arrowheads) of the connecting cilium. Scale bars = 1μm (d) IEM reveals SREB3, MAS1, and HTR1B at the basal body (BB) of the connecting cilium (CC) (arrowheads). Immunoelectron microscopy of MAS1 shows additional labelling throughout the CC and at the ciliary tip (arrow; two different images of photoreceptors are merged at the dashed line). Abbreviations: CE, centriole; IS, inner segment; OS, outer segment. Scale bars = 400nm.

Journal: Nature cell biology

Article Title: An siRNA-based functional genomics screen for the identification of regulators of ciliogenesis and ciliopathy genes

doi: 10.1038/ncb3201

Figure Lengend Snippet: (a) Localisation of selected indicated GPCRs (green) to proximal or basal regions of primary cilia (polyglutamylated α-tubulin; red) in differentiated SH-SY5Y neuronal cells. Magnified insets for selected cells (white arrowheads) are shown in white frames. Scale bar = 10μm. ( b ) Immunofluorescence (IF) labelling of MAS1 and OPRL1 (green) in the ciliary regions of photoreceptor cells (CR) (red; yellow arrowheads in magnified insets) in adult mouse retina. Abbreviations: GCL, ganglion cell layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer. Scale bar = 50μm ( c ) High magnification immunofluorescence for GPCRs (green) and centrin-3 (red) reveals localisation of GPR20, SREB3, and MAS1 at the adjacent centriole and the basal body (arrowheads) of the connecting cilium. Scale bars = 1μm (d) IEM reveals SREB3, MAS1, and HTR1B at the basal body (BB) of the connecting cilium (CC) (arrowheads). Immunoelectron microscopy of MAS1 shows additional labelling throughout the CC and at the ciliary tip (arrow; two different images of photoreceptors are merged at the dashed line). Abbreviations: CE, centriole; IS, inner segment; OS, outer segment. Scale bars = 400nm.

Article Snippet: The following antibodies were used with the indicated titres for immunofluorescent staining: rabbit anti-PRP6 (Santa Cruz sc-48786) 1:100, mouse anti-PRPF8 (Santa Cruz sc-55534) 1:100, rabbit anti-PRPF8 (Santa Cruz sc-030207) 1:100, goat anti-PRPF31 (Abnova PAB7154) 1:100, PIBF1/CEP90 (Novus NBP2-19823) 1:100, rabbit anti-PLK4 (Cell Signalling Technologies 3258) 1:100, rabbit anti-GPR20 polyclonal (Thermo Scientific, PA3-068) 1:100, rabbit anti-SREB3 (Novus Biologicals NBP1-71064) 1:100, rabbit anti-MAS1 (Novus Biologicals NBP1-78444) 1:100, rabbit anti-HTR1B (Novus Biologicals NBP1-01013) 1:100, rabbit anti-P2RY14 (Novus NBP1-70970) 1:100, rabbit anti-CRHR2 (Novus NBP1-00767) 1:100, rabbit anti-OPRL1 (Abcam ab66219) 1:50, rabbit anti-C21orf2 (GeneTex [N1C2], GTX119046) 1:100, mouse anti-acetylated alpha tubulin (Sigma Aldrich clone 6-11 B1 T6793) 1:1000, rat anti-ZO1 (1:400, Santa Cruz), rabbit anti-β-catenin (1:400, BD Bioscience), rabbit anti-gamma tubulin (AbCam ab11317) 1:1000, rabbit anti-ARL13B (Proteintech 17711-1-AP) 1:1000, mouse anti-GT335 monoclonal (Enzo Life Sciences ALX-804-885-C100) 1:1000 and mouse anti-FLAG (Sigma F3165) 1:1000.

Techniques: Immunofluorescence, Immuno-Electron Microscopy